Cultivo y fusion de protoplastos []. Loyola Vargas, V.M. Rosell, C.H. (ed.) Villalobos Arambula, V.M. (ed.) Access the full text: NOT AVAILABLE. Lookup the . Un protoplasto es una cĂ©lula de planta, bacteria u hongo que ha perdido total o parcialmente su pared celular, para lo cual se usan. Index terms: Citrus sp., gamma irradiation, grapefruit, protoplast fusion, somatic foi de protoplastos de ‘Ruby Red’ irradiados com 80 Gy, fusionados com.

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;rotoplastos select hybrids, regeneration of in vitro plants was subjected to RAPD analysis. Regeneration of fertile plants from Helianthus nuttalli and Helianthus giganteus L.

Abstract Interspecific somatic hybrids were obtained by polyethylene glycol fusion of protoplasts from tetraploid Solanum tuberosum L. Interspecific somatic hybridization between Solanum tuberosum L.

Somatic hybrids between potato Solanum tuberosum and wild species Solanum vernei fusij a recombination in the plastome. After seven days, the culture prootplastos was diluted 1: Somatic potato hybrids have been produced by fusion of diploid wild species with tetraploid S. Production and molecular characterization of Citrus intergeneric somatic hybrids between red tangerine and citrange.

Protoplast fusion between haploid gametic and diploid somatic cells has been achieved which clearly demonstrate that fusion is independent of cell type.

When colonies had developed, alginate beads were despolimeryzed using 20 mM sodium citrate solution in sorbitol 0,3 M, pH 7,4 and gently shaking. Introduction Potato, Solanum tuberosum L.

A rapid calli proliferation was observed and most of them showed purple-colored cells Fig. Protoplasts of both types are mixed in an equal proportion attaining a density of 5 protoplaetos 10 4 to 2 x 10 5 protoplasts per ml.

The ploidy of the genotype used in plant regeneration has been shown to profoundly affect the type of variation observed in morphological characters and chromosome numbers among regenerant plants. None of the plants had a similar banding pattern in relation to each one of the parents.


The numbers of chromosome of S.

Regeneration in Gewebekulturen einiger Dihaploider Solanum tuberosum Z. After that, protoplasts settled down. After that, colonies were dispersed into plates with solid MS13K regeneration medium Benke, Using chemical or electrical procedures Jones, protoplasts from different donor plants can be fused together and somatic hybrids regenerated from the fusion products. Improvements in regeneration from protoplasts of potato and studies on chromosome stability.

Universidad Nacional Agraria La Molina. For the obtained somatic hybrid plants, the fusinn number varied from Table 2.

Cultivo y fusion de protoplastos [1990]

Nineteen somatic hybrid plants were obtained from fusion experiments using PEG in an effort to combine elite traits from both parents. Solanum, protoplast fusion, polyethylene glycol, somatic hybrid, RAPD analysis. We describe here fusion between phospholipid vesicles prootoplastos and protoplasts to the fungus phycomyces blakesleeanus.

Plant material and protoplast isolation. The effect of initial culture media. Tissue culture procedures to produce somatic hybrids and the application of molecular biological tools for their analysis are well established.

Analysis of intra-specific somatic hybrids of potato Solanum tuberosum using simple sequence repeats. These samples were protoplastoos from rooted plants maintained in a greenhouse.

Protoplast fusion, culture and regeneration Prior to protoplsstos, protoplasts from S. The wild species S. The authors would like to thank the International Potato Center for financial support and to Martha Williams de Castro who revised the English version of this article. Nineteen somatic hybrid plants were obtained and confirmed by RAPD analysis. Then the suspension was centrifugated at rpm during ten minutes. Origins of cultivated potatoes and species relationships. Final concentration was protoplasts per millilitre.


The production of somatic hybrid plants with a chromosome number deviating from the expected is well documented in many fusion combinations where potato protoplasts have been used as one or both fusion parents Austin et al.

Plant regeneration from mono- and dihaploid potato genotypes often results in ploidy changes, but little aneuploidy; on the other hand, regenerated plants protlplastos tetraploid genotypes produce a wide range of aneuploidy Kumar, Protoplasts fusion from S. Somatic hybrids were confirmed morphologically under greenhouse conditions, when intermediate characteristics between parent lines, such as shoot coloration, leaf shape and pubescence were observed.

Isolation of plant DNA from fresh tissue. Fusion-treated protoplasts were cultured in V-KM medium supplemented with bovine serum albumin.

Fusion de Protoplastos by Arely Gutierrez on Prezi

After protoplasts fusion and during plant regeneration it frequently happens that genetic changes such as variations in chromosome number occur. Chromosome analysis The numbers of chromosome of S. Protoplasts were precipitated by centrifugation at rpm during five protopalstos. Both 6carboxyfluorescein and the kanamycin resistance harboured by the plasmid have been transferred from liposomes to protoplasts of phycomyces by the fusion technique.

This value is different from that obtained by Mattheij et al.

RAPD markers for confirmation of somatic hybrids in the dihaploid breeding of potato Solanum tuberosum L.

Somatoclonal variation in potato: An analysis of putative fusion products is essential to confirm hybrid status Masuelli et al. Prior to fusion, protoplasts from S.

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