/ Fractogel® EMD SO3 (S), (M). -. Ion Exchange chromatography using strong cation exchangers. Fractogel® ion exchangers are cross-linked. Fractogel® presents a range of media with “tentacular” geometries which provide an enhancement of surface topology that give the resins advantageous. Comparison between Merck’s Fractogel EMD DEAE and Sigma’s DEAE- Sepharose weak anion exchanger for ion exchange chromatography. I am purifying an.
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The separation mode is based on the differential penetration of molecules of different size and shape into the pores of the stationary phase.
Influence of packing velocity on column performance. Daniel Magiera 2 Estimated H-index: Chatziathanassiou University of Mainz. Packing of Toyopearl columns for gel filtration: New ion exchangers for the chromatography of biopolymers.
Import Data and Price of fractogel emd | Zauba
Determination of alkaline phosphatase aggregation by size exclusion high-performance liquid chromatography with low-angle laser light scattering detection. Particle size distribution was determined by two independent methods, coulter counting and SEM pictures, with fair agreement. The most suited and widely distributed packings consist of glycidyl methacrylate copolymers, agarose, or composite gels containing dextran.
Elution of lipoprotein fractions containing apolipoproteins E and A-I in size exclusion on Superose 6 columns is sensitive to mobile phase pH and ionic strength. Efficiency results present the expected trend of increasing dependence of plate height as a function of increasing flow rate, and the highest flow dependence was observed for Fractogel EMD COO. Dependence of optimal packing velocity on column size.
Influence of surface modification on protein retention in ion-exchange chromatography: View in Source Cite this paper.
Unger University of MainzM. Marina UrmannHeiner Graalfs. Jerker Porath 49 Estimated H-index: The effect of competitive binding from yeast fermentation components on dynamic binding capacity of aprotinin was studied showing a significant decrease in binding capacity.
Testing was done with three proteins: Chun-hua Cai 2 Estimated H-index: The methacrylate-based copolymer Fractogel EMD BioSEC used for large-scale purification of biological macromolecules is ejd to overcome the drawback derived from the poor pressure stability of soft gels.
Comparison of chromatographic ion-exchange resins V. Strong and weak cation-exchange resins.
Thus, gels made for SEC should have controlled range of size of the pores. Size-exclusion chromatography of plasma proteins with high molecular masses. Earle Stellwagen 33 Estimated H-index: Yoshio Kato 19 Estimated H-index: Other Papers By First Author.
Chromatography Methacrylate Molar mass distribution Organic chemistry Ionic strength Materials science Size-exclusion chromatography Macromolecule Polymer Agarose Glycidyl methacrylate.
Results of this paper may be used in the selection of resins for further testing in biopharmaceutical protein purification process development. Are you looking for One main advantage of SEC is that: Djuro Josic 27 Estimated H-index: Binding strength data of cation-exchange resins as a function of ionic strength depends on the protein, but binding and elution at high salt concentration may in general be performed with MacroPrep resins.
Strong and weak cation-exchangers were compared emv a number of chromatographic parameters, i. Cation-exchange chromatography of monoclonal antibodies: John Westerlund 1 Estimated H-index: Evaluation using different retention models journal of chromatography a [IF: