DDRT PCR PDF

(a) Principle of the reactions underlying DDRT-PCR analysis. (N) Any of the four nucleotides G, A, T, or C. V can be any nucleotide besides T. (B) Any nucleotide. Recently, a new approach has successfully been developed: Differential-Display Reverse Transcription-PCR (DDRT-PCR). This technique has been proven to. Establishment of infection and disease implies modifications in the genetic programmes of the cell systems that are involved and the differential expression of.

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CitePeer Related Articles http: Gene Ontology GO Terms. The products of endophytic bacterial isolate B.

Serine protease is an enzyme that produced by many organisms including bacteria. Of the eight compounds identified in the inhibition zone, the prevailing compounds were dodecane However, only one of the sequenced genes was matched with genes deposited in GenBank. Verma cdrt Verma reported the isolation and purification of antimicrobial peptide from celomic fluid of Indian earthworm Pheretima posthumous.

Earthworm- a potential source for stable and potent antimicrobial compounds- isolation and purification study. The extraction of the active metabolites produced by the endophytic isolate SA was done as follows: Differential display can offer some favors regarding this point, especially in the identification of some up-regulated or down-regulated genes under specific conditions. In-vivo expression technologies and differential display RT-PCR are providing new approaches to further examine a microbe’s response to experimental conditions which more closely resemble natural microbial associations and habitats.

On the other hand, only chloramphenicol recorded 2. One of the rdrt was chosen and transferred to a new NA plate to ensure complete purification. Acknowledgments We would like to thank King Khalid University, Faculty of Science, Biology Department for supporting this work with all the required chemicals and equipments. Differential display using Ea1 and Ea2 primers combined with OP1 reverse transcription primer.

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Arbuscular mycorrhizal fungi increased growth, nutrient uptake and ddtt to salinity in olive trees under nursery conditions. In vitro antifungal activity of Argemone ochroleuca Sweet latex against some pathogenic fungi.

Moustafa1, 2 Tarek H. Articles from 3 Biotech are provided here courtesy of Springer. Materials and methods Plant materials Salvadora persica L. Biocontrol of plant disease: Antibacterial activity of volatile component and various extracts of Spirulina platensis. The sterilized cubic stem parts were then divided into two pieces using a sterilized razor blade. A typical GC—MS chromatogram of the ethanol extracts inhibition zone slices.

In the latter case, please turn on Javascript support in your web browser and reload this page. The antibacterial activity of the chosen bacterial isolate, named SA, was examined against S.

Differential display – Wikipedia

Plant surface sterilization is necessary to avoid the co-contamination with superficial bacteria. Antibacterial effects of antibiotics compared with endophytic isolate SA against S. Although chloramphenicol showed higher activity than our endophytic isolate; the antagonistic activity of this isolate is more preferred than this antibiotic.

Compliance with ethical standards Conflict of pc All authors declare no conflict of interest. However, we strongly propose that our isolate was able to produce serine protease for antimicrobial purpose. Both of sterile filter paper discs were loaded with the isolate SA and ten different antibiotic discs.

The bacterial production and secretion of serine protease for antimicrobial function is not yet documented. Antagonistic effect of endophytic isolate SA against S. Chitinolytic activity of fdrt endophytic strain of Bacillus cereus.

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Differential display

The purified bacterium was checked for its ability to antagonize some human pathogenic bacteria including Klebsiella pneumoniaeEscherichia coli and S. The mixture was filtered using filter papers to remove the agar medium pieces and the ethanolic filtrate was undergoing to GC—MS analysis as described previously Ezhilan and Neelamegam ; Moustafa pxr al. Application of differential display RT-PCR revealed that the isolate was able to up-regulate a gene with serine protease like protein.

National Center ddrtt Biotechnology InformationU.

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Decane, tetradecane, pyrrolo [1,2-a] pyrazine-1,4-dione, hexahydro- and 2H-Chromenecarboxamide, 8-allyloxo- N -[2- 2-methyl-1H-indolyl ethyl]-found to be the minor components 4. GC—MS chromatogram analysis of the ethanol extract of inhibition zone parts s howed eight peaks that led to the identification of a number of compounds Fig.

This gene was ddft, excised from the gel and submitted for gene sequencing Fig.

Impact of isolate SA activity in comparison with different antibiotics The antagonistic activity of the endophytic bacterial isolate was compared with ten different known bacterial antibiotics against S. In this study, Bacillus endophyticus strain SA isolated from the inner tissue of the stem of the cultivated plant Salvadora persica, Asir, Kingdom of Saudi Arabia produces an antagonistic factor. Extraction of the bioactive metabolites produced by SA isolate The extraction of the active metabolites produced by the endophytic isolate SA was done as follows:

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