Thermo Scientific. HILIC Separations Technical Guide. HILIC. Separations. A Practical Guide to HILIC Mechanisms, Method. Development and Troubleshooting. A Practical Guide to HILIC ISBN Authors: Patrik Appelblad, Tobias Jonsson, Einar Pontén, Camilla Viklund and Wen Jiang Editor: Tobias. () A Practical Guide to HILIC. Se Quant, Sweden. equant. com. has been cited by the following article: TITLE: Optimization and.
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Practical Approaches to Peptide Isolation. For ion pairing chromatography, the concentration z pentafluoropropionic acid was tested and the greatest value appeared to be 9. An analytical method for the separation and detection of 12 aminoglycosides has been optimized using two kinds of chromatographic conditions HILIC, Ion pairing.
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Comprehensive Guide to HILIC – Hydrophilic Interaction Chromatography : Waters
Their ionic character, the similarity structures and the high polarity due to the presence of two or more amino and hydroxyl groups cause a difficulty in separation and make these compounds poorly retained on the reversed phase column. We ask, “how’d you do it? View the complete course list. Discount will be applied to cart at checkout. MS Imaging for Biomedical Research Understand molecular visualization and spatial distribution of compounds.
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Therefore, the comparison between the two separation methods shows that the response area of the majority of analytes tested increases when using the ion pair giide.
Comprehensive Guide to HILIC – Hydrophilic Interaction Chromatography 
Search Waters KnowledgeBase – find answers to your troubleshooting questions. The ‘Comprehensive Guide to Hydrophilic Interaction Chromatography’, a page book, illustrates how HILIC works and how separation scientists can improve their success in separating and quantifying polar compounds in a variety of sample matrices.
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The maximum and adequate concentration of ammonium acetate for the majority of analytes was set to 30 mM. Increase detection selectivity and confidence using mass detection.